Protein Expression Specialist
Vor 6 Tagen
We are seeking a highly skilled Protein Expression Specialist to join our team at the Botnar Institute of Immune Engineering (BIIE). The successful candidate will be responsible for leading efforts in soluble protein expression, high-throughput protein expression systems, and cell line development for stably expressing cell lines.
Key Responsibilities- Design, develop, and optimize expression strategies for recombinant proteins using bacterial, insect, and mammalian expression systems.
- Purify recombinant proteins using chromatography techniques (e.g., affinity, ion exchange, size exclusion), troubleshoot purification processes, and ensure the production of high-quality proteins.
- Lead high-throughput antibody production efforts, including transient and stable transfection, expression screening, and large-scale production of antibodies.
- Establish and maintain cell lines for protein expression, including stable cell line generation, clone selection, and characterization for consistent protein yield.
- Analyze protein expression and purification data, optimize protocols for yield and quality, and maintain detailed experimental records.
- Ensure all protein production and purification processes comply with quality control standards, maintaining rigorous attention to detail and data integrity.
- Collaborate with cross-functional teams to support research development projects and manage multiple projects simultaneously, ensuring timely delivery of high-quality proteins for various research programs.
- PhD in Molecular Biology, Biochemistry, Biotechnology, or a related field. Candidates with a Master's degree and significant industry experience may also be considered.
- Strong expertise in recombinant protein expression and purification, particularly using bacterial, insect, and mammalian systems.
- Experience with high-throughput protein or antibody production, including transient and stable expression in mammalian cells.
- Proficiency in chromatographic purification techniques and protein characterization methods (e.g., SDS-PAGE, Western blot, HPLC, mass spectrometry).
- Familiarity with cell line development and culture techniques, including experience in stable cell line generation.
- Strong communication skills, both written and verbal, with the ability to present complex scientific data to diverse audiences.
About the Botnar Institute of Immune Engineering
The Botnar Institute of Immune Engineering (BIIE) is a non-profit research institute focusing on developing translational solutions for treatment, prevention, and diagnostics of diseases relevant in global child and adolescent health. We are committed to advancing the field of Immune Engineering through interdisciplinary research and collaboration.
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