PhD student position: deciphering the molecular basis of allostery in CRISPR associated proteins using single-molecule experiments

vor 3 Wochen


Basel, Schweiz The Biozentrum, University of Basel Vollzeit

Your position



This PhD project will grow with you. You will create time-resolved direct observations of single CARF proteins – aka. CRISPR suicide proteins – undergoing allosteric activation, substrate processing, deactivation, etc. How this works, on what timescales, by which conformational rearrangements has not been observed yet in real-time, leaving the underlying molecular processes obscure. In this PhD project, you'll be able to solve (some of) these nanoscale puzzles. Steps involved in the project: Single-molecule fluorescence/FRET/DyeCycling experiments Solid-state nanopore/NEOtrap experiments Advanced data analysis, coding, kinetic modeling Protein production and functionalization

Your profile

Prior experience in several of these areas is desired:

Single-molecule experiments and interpretation Advanced data analysis, coding, modeling, machine learning Optics and fluorescence microscopy and/or nanopore techniques Protein biochemistry
Essential requirements:

MSc degree in physics or (bio-)chemistry with excellent grades High motivation and curiosity about fundamental questions on the physical-chemistry of life Independent analytical thinking and systematic work style Excellent experimental problem-solving skills and perseverance Friendly team player proficient in English communication (speaking & writing)

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