Phd Position in Appl I Ed Immunology
Vor 7 Tagen
Selected publications from our laboratory relevant to this project: ( i ) Ca2+ signaling
through ryanodine receptor 1 enhances maturation and activation of human dendritic cells. Bracci et al. (2007) _J. Cell Sci._ **120**:2232-2240. (ii) Ryanodine receptor activation by Cav1.2 is involved in dendritic cell MHC class II surface expression. Vukcevic et al. (2008). _J. Biol. Chem._ **283**:34913-34922. (iii) Frequent calcium oscillations lead to NFAT activation in human immature dendritic cells. Vukcevic et al. (2010)._ J. Biol. Chem_ **285**:16003-16011. (iv) Gain of function of the immune system caused by a ryanodine receptor 1 mutation. Vukcevic et al. (2013). _J. Cell Sci._ **126**:3485-3492. (v) Functional characterization of endogenously expressed human RYR1 variants.Z orzato, et al. (2021). _J. Vis. Exp._ (**172**), e62196.
**Your position**
a) Manage and functionally characterize EBV-immortalized human B-lymphocyte cell lines from patients with _RYR1_ mutations and non-mutant family members.
b) Compare the functional effect of novel _RYR1_ mutations in muscle cells and EBVimmortalized human B-lymphocytes.
c) Profile sera from _RYR1_ mutation carriers and non-mutant family members for cytokine content at rest and after vaccination.
d) Assess the functional parameters of the innate and adaptive immune system of patients with _RYR1_ mutations compared to non-mutant family members.
**Your profile**
**We offer you**
A stimulating, challenging and interdisciplinary research environment, state-of
- the-art
technologies and core facilities, and attractive employment conditions. The** DBM is an international institute** pursuing basic, translational and clinical research, with access to cutting-edge core facilities.
**Application / Contact
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