Junior Scientist
Vor 4 Tagen
**Our Client**
**Junior Scientist (m/w)****:
**Your responsibilites**
- In close collaboration with biology/chemistry group leaders and scientists design, develop, execute and analyze a variety of experiments, including but not limited to:
- RNA/DNA isolation, RT-PCR, electrophoresis, RT-qPCR for analysis of gene expression and detection of splice variants;
- Mammalian cell culture, transfection/transduction, generation of stable cell lines;
- Cell-based assays including luminescence/fluorescence reporter assays, imaging, FACS, proliferation and viability assays
- Protein extraction, immunoblotting, immunoprecipitation, ELISA, protein assays
- Small molecule testing in miniaturized assay format
- Record all data and results in electronic format with accuracy and responsibility in a timely manner
- Analyze and interpret results from a variety of readouts, including DNA/RNA sequence analysis
- Prepare presentations and efficiently discuss results at internal and external meetings
**Your profile**
- Technical Diploma, or a degree in molecular biology, biochemistry or related field (Bachelor or Master or PhD; with minimum 5-8 years of experience in an equivalent position, preferably within an industry context (collaboration or internships)
- Experience with drug discovery and compounds in oncology
- Excellent understanding of molecular biology and cellular assay approaches is required, including design of tools (e.g., qPCR primers and probes sets) and data analysis (e.g., sequence alignments, curve fitting)
- You are familiar with the common molecular biology techniques such as: RNA/DNA isolation, RT-PCR, electrophoresis, transfection/transduction, generation of stable cell lines, rt-qPCR and for gene expression analysis
- Good knowledge with cell based assays such as including luminescence/fluorescence reporter assays, imaging, FACS, proliferation and viability assays, immunoblotting, immunoprecipitation, ELISA, MSD and other protein assays
- Proficiency in MS Office package and GraphPad prism
- Fluent in English (spoken and written), any further languages are an asset
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