Internship - Quick Protein Content Quantification

vor 3 Wochen

CorsiersurVevey, Schweiz Merck Group Vollzeit

Work Your Magic with us

Ready to explore, break barriers, and discover more? We know you’ve got big plans - so do we Our colleagues across the globe love innovating with science and technology to enrich people’s lives with our solutions in Healthcare, Life Science, and Electronics. Together, we dream big and are passionate about caring for our rich mix of people, customers, patients, and planet. That's why we are always looking for curious minds that see themselves imagining the unimaginable with us.

United As One for Patients, our purpose in Healthcare is to help create, improve and prolong lives. We develop medicines, intelligent devices and innovative technologies in therapeutic areas such as Oncology, Neurology and Fertility. Our teams work together across 6 continents with passion and relentless curiosity in order to help patients at every stage of life. Joining our Healthcare team is becoming part of a diverse, inclusive and flexible working culture, presenting great opportunities for personal development and career advancement across the globe.

**Start date: 01.02.2025**

**Duration: 6 months**

**Location: Corsier-sur-Vevey**

**Role: Internship**

**Your role**:
As part of the Technology and Innovation team for Global Drug Substance Development, you will join a team of motivated scientists dedicated to developing and implementing solutions to support the development and manufacturing of new biological therapeutics. Within this group, you will contribute to the improvement of the enabling technologies to support the upstream process development and clinical manufacturing.

Goal of the internship project is to design a quick protein quantification approach using robotics and Process Analytical Technology (PAT).
- First part of the internship will be to finalize the evaluation of the ability of Raman spectroscopy and chemometrics to quantify protein concentration both at clinical manufacturing scale (2000L) and at process development scale (AMBR250 robotic platform).
- Second part (if relevant) will focus on designing a script on the AMBR250 liquid handler part to prepare a plate for quantification using an offline rapid analytical method (i.e. Octet). It may include protein quantification method development as well (tbc)

Your work may include:

- Designing and performing spiking experiments in the lab to generate Raman data for modeling protein quantification (incl. adaptation of existing script for in AMBR250 software)
- Performing Raman data analysis, including model calibration and validation using chemometrics (such as Partial Least Square multivariate data analysis)
- Evaluating Raman model scalability between robotic (AMBR 250) and small (3.5L) scales used in process development, potentially also with manufacturing scale.
- Evaluating AMBR250 liquid handler ability to prepare plates for offline protein content quantification, including scripting
- (possibly protein quantification method development)

You will be trained to be autonomous in the lab and in data treatments, will participate in the experimental design and in every discussion concerning your project.

**Your profile**:

- Master/engineer student in Biotechnology or other related fields
- Highly motivated and autonomous
- Ability to conceptualize, solve complex challenges and to communicate about your project
- Great team spirit
- Previous experience in data modeling and/or scripting is a plus
- Good written and oral knowledge in English, French is a plus

**What we offer**:We are curious minds that come from a broad range of backgrounds, perspectives, and life experiences. We celebrate all dimensions of diversity and believe that it drives excellence and innovation, strengthening our ability to lead in science and technology. We are committed to creating access and opportunities for all to develop and grow at your own pace. Join us in building a culture of inclusion and belonging that impacts millions and empowers everyone to work their magic and champion human progress

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