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PhD Student (Directed evolution and protein/enzyme engineering)
80 % A PhD project is available in the group of Prof. Dr. Alexandria (Ali) Deliz Liang in the Department of Chemistry at the University of Zurich (Switzerland). Our laboratory works on advanced methods in protein engineering including genetic code expansion. Currently, we are seeking a highly motivated PhD student to work on development and application of a novel protein engineering method.
Your responsibilities The successful candidate will
- clone plasmids for protein expression,
- optimize protein expression and purification methods,
- develop assays for screening enzymatic activity (absorbance, fluorescence, HPLC-MS),
- perform high-throughput expression and screening of enzyme variants,
- engineer enzymes using directed evolution, and
- characterize the engineered enzymes.
Your profile
- A highly motivated student with an MS degree in biochemistry, chemistry, or related field
- Enthusiastic about learning and applying new techniques
- Essential experience: cloning, protein expression and purification, enzyme assays
- Beneficial experience: cell-free protein expression, enzyme assay development, directed evolution, protein engineering
- Excellent communications skills in English (spoken and written) are essential
- Conscientious and inclusive working style
Place of workUniversity of Zurich Irchel, Winterthurerstrasse, Zürich, Switzerland
Start of employment The employment start date will be mutually agreed upon with the applicant and PI (approximate timeline between June 2024 and January 2025).
Applicants should submit a single PDF document that includes the following: i) a cover letter describing previous research and motivation for applying to this position, ii) a detailed curriculum vitae, and iii) the names of two people who can supply reference letters. Applications should be submitted via the link below. Applications will be assessed on a rolling basis until 01 September 2024. Given the rolling assessment of applications, candidates are encouraged to apply early.
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